RNA

Part:BBa_K1824564:Design

Designed by: Wenbo Xu   Group: iGEM15_XJTLU-CHINA   (2015-09-06)


RNA Thermometer U6 (Specially designed for T7)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 25
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 25
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To maintain the secondary structure of RNA thermometers, researchers need to be careful with of the use of any parts that couple with it. For instance, in some cases, part of operator region can be transcribed into RNAs and form tight secondary structure and thus interfere RNA thermometers.


Source

The DNA sequence of U6 was from registry.


References